Address: 125 Greenberg, University Park, PA 16803
Description: This facility houses a Kintek SX 300 stopped flow spectrometer (SFS) state-of-the art instrument equipped with an excitation light source coupled with a monochromator, two emission channels for fluorescence (photomultipliers) and an absorbance photodiode. A shutter as well as an ability to interface light into reactions has been custom designed for this instrument. Applications for this customizable SFS include:
- Enzyme kinetics
- Nanoparticle catalyst based kinetic measurements
- Fluorescence anisotropy
- Simple chemical kinetics
- Liposome and polymersome based membrane protein transport measurments (water and ions)
- Light driven ion transport measurements in rhodopsins
The SF-300X stopped-flow has a powerful, computer-controlled servo motor drive that provides precise and reproducible mixing. Double mixing experiments can be performed easily while consuming minimal volumes of precious samples and affording a miniml fast reaction time of only 5 milliseconds. The brushless high performance motor is digitaly controlled with a high torque, low inertia, digital power supply to essentially provide instantaneous starts and stops of the drive syringes.
Fluoresence - Absorbance Measurements
The SF-300 stopped-flow uses interchangeable observation cells. A choice of four observation cells is available, and each can record either absorbance or fluorescence or both simultaneously.
The instrument has the capability to produce a dead time of 0.85 milliseconds
Simple Sample-Load Volume
A unique two-position valve is used to allow easy and efficient sample loading. In one position, the three syringes are connected to their individual sample load ports. In the other position, the three syringes can be driven to force the mixing of reactants into the observation cell. A 20 microliter minimum sample volume is required to perform the analysis.
Sample submission: Newer applications are welcome and personnel involved in working with this instrument are eager to push the boundaries of this instrument and its capabilities. Please contact the laboratory to inquire about analytical capabilitites and method development.
Training: Student researchers and faculty who may be interested in training aon instrument operation and data interpretation, please contact the SFS laboratory to schedule training and time on the instrument. While the number of training hours may vary, depending on the student's knowledge and analytical skills, we require a minimum of six training hours prior to allowing the student to operate the instrument.